Immunoaffinity column for mycotoxins, its problems and solutions
نویسندگان
چکیده
منابع مشابه
Validation of an UHPLC-MS/MS method for simultaneous analysis of 11 mycotoxins in wheat flour Using Immunoaffinity column
This study focuses on optimization and validation of an Ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous analysis of 11mycotoxins: Aflatoxin B1, T-2 Toxin, Ochratoxin A, Deoxynivalenol and Zearalenone in wheat matrix. Sample extraction and cleanup procedure is based on a single extraction step using acetonitrile/water/acetic acid mixture...
متن کاملValidation of an UHPLC-MS/MS method for simultaneous analysis of 11 mycotoxins in wheat flour Using Immunoaffinity column
This study focuses on optimization and validation of an Ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method for simultaneous analysis of 11mycotoxins: Aflatoxin B1, T-2 Toxin, Ochratoxin A, Deoxynivalenol and Zearalenone in wheat matrix. Sample extraction and cleanup procedure is based on a single extraction step using acetonitrile/water/acetic acid mixture...
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Importance of herbal medicines have recently increased owing to rising interest in their health benefits. However, medicinal plant extracts are complex mixtures of phytochemicals that act synergistically or additively on specific and/or multiple molecular and cellular targets. Thus, it is difficult to examine the actual pharmacological roles of active compounds in plant extracts. This review de...
متن کاملsurvey of mycotoxins in wheat from iran by hplc using immunoaffinity column cleanup
mycotoxins are small, toxic chemical products produced as secondary metabolites by a few fungal spices that readily colonise crops and contaminate them with toxins in the field or after harvest. in this study a liquid chromatographic method was developed for the simultaneous determination of aflatoxins (afs) (b 1 , b 2 , g 1 , and g2), ochratoxin a (ota) and zearalenone(zea) toxins in wheat. fo...
متن کاملIsolation and concentration of Salmonellae with an immunoaffinity column.
A method for rapidly and selectively isolating Salmonellae from buffer solutions and concentrating the bacteria by a factor of approximately 500 was developed. Anti-Salmonellae antibody was covalently linked to 40 microm polyacrylamide beads to prepare a solid phase with affinity for the bacteria. The beads were packed into 1-mm diameter glass tubes to form a column 20 microl in volume. Buffer ...
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ژورنال
عنوان ژورنال: Mycotoxins
سال: 2003
ISSN: 0285-1466,1881-0128
DOI: 10.2520/myco1975.2003.suppl3_249